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Literature summary for 2.8.2.2 extracted from
- Hydroxylated and sulfated metabolites of commonly occurring airborne polychlorinated biphenyls inhibit human steroid sulfotransferases SULT1E1 and SULT2A1 (2018), Environ. Toxicol. Pharmacol., 58, 196-201 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of the enzyme in Escherichia coli strain BL21(DE3) | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,2',5,5'-tetrachloro-4-biphenylsulfate | - |
Homo sapiens | |
| 2,2',5,5'-tetrachloro-4-hydroxybiphenyl | - |
Homo sapiens | |
| 2,3',4-trichloro-4'-biphenylsulfate | - |
Homo sapiens | |
| 2,3',4-trichloro-4'-hydroxybiphenyl | - |
Homo sapiens | |
| 2,4'-dichloro-4-biphenylsulfate | - |
Homo sapiens | |
| 2,4'-dichloro-4-hydroxybiphenyl | - |
Homo sapiens | |
| 3,3'-dichloro-4-biphenylsulfate | - |
Homo sapiens | |
| 3,3'-dichloro-4-hydroxybiphenyl | - |
Homo sapiens | |
| 4-chloro-4'-biphenylsulfate | - |
Homo sapiens | |
| 4-chloro-4'-hydroxybiphenyl | - |
Homo sapiens | |
| additional information | hydroxylated and sulfated metabolites of commonly occurring airborne polychlorinated biphenyls inhibit human steroid sulfotransferases SULT1E1 (EC 2.8.2.4) and SULT2A1. No inhibition by 3,3'-dichloro-4-biphenylsulfate at up to 0.01 mM | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylyl sulfate + dehydroepiandrosterone | Homo sapiens | - |
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
? |  |
| additional information | Homo sapiens | SULT2A1 catalyzes the sulfation of hydroxysteroids such as dehydroepiandrosterone and various alcohol-containing androgens | ? | - |
- |
|
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q06520 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli strain BL21(DE3) | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylyl sulfate + dehydroepiandrosterone | - |
Homo sapiens | adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
? | |
| additional information | SULT2A1 catalyzes the sulfation of hydroxysteroids such as dehydroepiandrosterone and various alcohol-containing androgens | Homo sapiens | ? | - |
- |
|
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alcohol/hydroxysteroid sulfotransferase | - |
Homo sapiens |
| steroid sulfotransferase | - |
Homo sapiens |
| SULT2A1 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | inhibition kinetics | Homo sapiens |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0013 | - |
recombinant SULT2A1, pH 7.4, 37°C | Homo sapiens | 2,2',5,5'-tetrachloro-4-hydroxybiphenyl | |
| 0.0015 | - |
recombinant SULT2A1, pH 7.4, 37°C | Homo sapiens | 2,4'-dichloro-4-hydroxybiphenyl | |
| 0.0049 | - |
recombinant SULT2A1, pH 7.4, 37°C | Homo sapiens | 2,3',4-trichloro-4'-hydroxybiphenyl | |
| 0.012 | - |
recombinant SULT2A1, pH 7.4, 37°C | Homo sapiens | 4-chloro-4'-hydroxybiphenyl | |
| 0.023 | - |
recombinant SULT2A1, pH 7.4, 37°C | Homo sapiens | 3,3'-dichloro-4-hydroxybiphenyl | |
| 0.03 | - |
recombinant SULT2A1, pH 7.4, 37°C | Homo sapiens | 2,2',5,5'-tetrachloro-4-biphenylsulfate | |
| 0.046 | - |
recombinant SULT2A1, pH 7.4, 37°C | Homo sapiens | 2,4'-dichloro-4-biphenylsulfate |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | both SULT1E1 and SULT2A1 can play important roles in steroid signaling through regulation of the active forms of the hormones | Homo sapiens |
| physiological function | SULT2A1 catalyzes the formation of dehydroepiandrosterone sulfate (DHEAS) the most abundant circulating steroid in humans. DHEAS serves as a transport form for dehydroepiandrosterone (DHEA) in the serum. Following uptake of DHEAS by tissues and its subsequent hydrolysis catalyzed by steroid sulfatase, DHEA serves as a precursor for both androgens and estrogens | Homo sapiens |
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